Test of the Effectiveness of Bilalo Sea Cucumber (Actinopyga Mauritiana) in Pancreatic Beta Cell Repair in Aloxan-Induced Mice (Mus Musculus)

Anita Aris(1*), Ermi Girsang(2), Ali Napiah Nasution(3)
(1) Department of Biomedical Science, Faculty of Medicine, Universitas Prima Indonesia
(2) Department of Biomedical Science, Faculty of Medicine, Universitas Prima Indonesia
(3) Department of Biomedical Science, Faculty of Medicine, Universitas Prima Indonesia
(*) Corresponding Author
DOI : 10.30604/jika.v8i3.1982

Abstract

Introduction diabetes mellitus (DM) is a degenerative disease that occurs due to abnormal carbohydrate metabolism. DM conditions occur when the pancreas is unable to produce insulin or the sensitivity of cell receptors to insulin is very low. The increase in DM occurs when the pancreatic beta cells are unable to produce insulin or the sensitivity of cell receptors to insulin is very low. Damage to pancreatic beta cells can be caused by diabetogenic substances such as alloxan. Teripang bilalo (Actinopyga mauritiana) is a marine animal that has been used as food and medicine. Contains many secondary metabolites, namely flavonoids, alkaloids, glycosides, steroids, and saponins. active compound flavonoids and saponins., research objectives to know the effectiveness of oral administration of teripang billion in reducing blood sugar levels and the ability to repair tissue in pancreatic beta cells seen from hispatology, materials, and methods is an experimental study with 25 mice as subjects. Oral administration of sea teripang bilalo ethanol extract at doses of 100, 200, and 400 mg/kg, positive control by oral administration of glibenclamide, and negative control by administration of Na-CMC then a histopathological examination of the pancreas was performed, research results in the administration of teripang bilalo ethanol extract 400 mg / kg proved to be able to reduce blood sugar levels to 179.4 mg / dl and proved to have an effect on the improvement of pancreatic histology, which is a picture of damage to peripheral islands of langerhans by 1, damage to islands of langerhans less than 50% by 2.4 and damage, conclusions administration of teripang bilalo ethanol extract is proven to reduce blood sugar levels and have an effect on improving pancreatic histology.

Keywords


Diabetes mellitus (DM); teripang bilalo; Actinopyga mauritiana; blood sugar levels; pancreatic histology

References


Aksara, R., Musa, W. J. A. and Alio, L. Identification of alkaloid compounds from methanol extract of mango bark (Mangifera indica L). Journal of Entropy, III, 2013; Pp. 514–519.

American Diabetes Association. Standards Of Medical Care Standards of Medical Care in Diabetes d 2016. Edited by C. William T. USA: American Diabetes Association. 2016.

Gunawan, S. Pharmacology and Therapeutics. 5th edition. Edited by R. Setiabudy, Nafrialdy, and Elysabeth. Jakarta: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia. 2007.

Hawa, J. I. and al. The potential of the coelomic fluid in sea cucumber as an antioxidant, Malays J Nutr., 1999; 5(1), pp. 55–9.

Karnila, R. Utilization of Sea Cucumber Bioactive Components in the Health Sector, University of Riau Repository. 2011.

Mamelona, J. et al. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber, Cucumaria frondosa’, Food Chemistry, 2007; 104, pp. 1040–1047.

Martoyo, J. Sea cucumber cultivation. Edited by N. Aji and T. Winanto. Jakarta: Self-help Spreaders. 2000.

Ministry of Health of the Republic of Indonesia. Basic Health Research. 2013.

Ministry of Health of the Republic of Indonesia. Data Center and Information of the Ministry of Health of the Republic of Indonesia. Jakarta: Data and Information Center, 2014; p. 2.

Mustikasari, K. and Ariyani, D. Study of the potential of Binjai (Mangifera caesia) and Kasturi (Mangifera casturi) as antidiabetics through phytochemical screening in roots and stems, Science and Applied Chemistry, 2008; 2(2), pp. 64–73.

Sabir, A. Antibacterial activity of flavonoid propolis Trigona sp against Streptococcus mutans bacteria (in vitro), Maj. Ked. Gigi (Dent. J.), 2005. Pp. 135–141.

Wresdiyati, T. et al. 'Sand Sea cucumber Increases Antioxidant Content of Superoxide Dismutase in Pancreatic Diabetic Rats', Veterinary Journal March 2015, 2015; 16(1), pp. 145–151.


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