Memory Loss induced by Aspartame in Albino Rats: Study on neurobehavioral changes

Vicko Suswidiantoro(1), Fadlina Chany Saputri(2*), Abdul Mun'im(3)
(1) Universitas Indonesia
(2) Universitas Indonesia
(3) Universitas Indonesia
(*) Corresponding Author
DOI : 10.30604/jika.v6i2.511


Objective: Aspartame (ASP) consumption in various food and beverage products has generated a lot of controversy on safety. Many reports, ASP caused deterioration of health condition likes diabetes, psychiatric disorders, memory loss, etc. This study aimed to investigate the optimization duration of ASP to induce memory loss in Sprague Dawley rats. Methods: The ASP was administered 40 mg/kg BW orally for 28 days in rats. Analysis of memory loss by neurobehavioural changes including latency time, length of track, per cent time and frequency target quadrant using Morris Water Maze (MWM) at day 14, 21, 28, and 24 hours after the last treatment. Results: The administration of ASP showed the time-dependent changes for each indicator of neurobehavioural. The results demonstrated during 28 days of induction showed a significant decrease in latency time, length of track, per cent time and frequency target quadrant. Conclusion: From the results, it can be concluded administration of ASP during 28 days induce neurobehavioural changes related to memory loss in rats.



Abstrak: Objektif: Penggunaan aspartame (ASP) sebagai pemanis buatan yang banyak terdapat pada makanan, dan minuman menimbulkan berbagai kontroversi dalam hal keamanan. Berbagai data menunjukkan ASP dapat menyebabkan gangguan kesehatan seperti diabetes, gangguan psikiatrik, penurunan fungsi memori, dll. Penelitian ini bertujuan untuk menentukan waktu durasi penggunaan ASP yang dapat menyebabkan gangguan fungsi memori pada tikus Sprague Dawley. Metode:ASP diberikan secara oral pada tikus dengan dosis 40 mg/kg BB selama 28 hari. Analisis terhadap fungsi memori dilakukan dengan adanya perubahan perilaku pada hewan, meliputi waktu latensi, Panjang lintasan, persentase waktu dan frekuensi kuadran menggunakan Morris Water Maze (MWM) setelah 24 jam setelah pemberian pada hari ke 14,21, 28. Hasil: Pemberian ASP menunjukkan adanya perbedaan waktu pada setiap indicator perilaku. Pemberian selama 28 hari menujukkan adanya perbedaan signifikan pada penurunan waktu latensi, Panjang lintasan, persentase waktu dan frekuensi kuadran. Kesimpulan: Berdasarkan hasil, pemberian ASP selama 28 hari dapat menyebabkan adanya perubahan perilaku yang berkaitan dengan fungsi memori pada tikus.


Aspartame; Memory loss; Neurobehavioral; Morris Water Maze; Fungsi memori; Perilaku; Morris Water Maze


Abdel-Salam, O. M., Salem, N. A., El-Shamarka, M. E., Hussein, J. S., Ahmed, N. A., & El-Nagar, M. E. (2012). Studies on the effects of aspartame on memory and oxidative stress in brain of mice. European Review for Medical and Pharmacological Sciences, 16(15), 2092–2101.

Anand, K., & Dhikav, V. (2012). Hippocampus in health and disease: An overview. Annals of Indian Academy of Neurology, 15(4), 239–246.

Choudhary, A. K., & Pretorius, E. (2017). Revisiting the safety of aspartame. Nutrition Reviews, 75(9), 718–730.

Ezzati, A., Katz, M. J., Zammit, A. R., Lipton, M. L., Zimmerman, M. E., Sliwinski, M. J., & Lipton, R. B. (2017). Differential Assocition of Left and Right Hippocampal Volumes With Verbal Episodic and Spatial Memory in Older Adults. Neuropschologia, 93, 380–385.

Guy, R. C. (2014). Aspartame. Encyclopedia of Toxicology: Third Edition, 1(330), 330–331.

H. Ferreira-Vieira, T., M. Guimaraes, I., R. Silva, F., & M. Ribeiro, F. (2016). Alzheimer’s disease: Targeting the Cholinergic System. Current Neuropharmacology, 14(1), 101–115.

Humphries, P., Pretorius, E., & Naudé, H. (2008). Direct and indirect cellular effects of aspartame on the brain. European Journal of Clinical Nutrition, 62(4), 451–462.

Iyyaswamy, A., & Rathinasamy, S. (2012). Effect of chronic exposure to aspartame on oxidative stress in brain discrete regions of albino rats. Journal of Biosciences, 37(4), 679–688.

Kandel, E. R., Dudai, Y., & Mayford, M. R. (2014). The molecular and systems biology of memory. Cell, 157(1), 163–186.

Kumar, K., Kumar, A., Keegan, R. M., & Deshmukh, R. (2018). Recent advances in the neurobiology and neuropharmacology of Alzheimer’s disease. Biomedicine and Pharmacotherapy, 98(December 2017), 297–307.

Lindseth, G. N., Coolahan, S. E., Petros, T. V., & Lindseth, P. D. (2014). Neurobehavioral effects of aspartame consumption. Research in Nursing and Health, 37(3), 185–193.

Magnuson, B. A., Burdock, G. A., Doull, J., Kroes, R. M., Marsh, G. M., Pariza, M. W., … Williams, G. M. (2007). Aspartame: A safety evaluation based on current use levels, regulations, and toxicological and epidemiological studies. Critical Reviews in Toxicology, 37(8), 629–727.

Nabors, L. O. (2001). Alternative Sweeteners (Third Edit). New York: Marcel Dekker, Inc.

O’Mullane, M., Fields, B., & Stanley, G. (2014). Food Additives: Sweeteners. Encyclopedia of Food Safety, 2(22839), 477–484.

Onaolapo, A. Y., Onaolapo, O. J., & Nwoha, P. U. (2016). Alterations in behaviour, cerebral cortical morphology and cerebral oxidative stress markers following aspartame ingestion. Journal of Chemical Neuroanatomy, 78, 42–56.

Peña-Bautista, C., Baquero, M., Vento, M., & Cháfer-Pericás, C. (2019). Free radicals in Alzheimer’s disease: Lipid peroxidation biomarkers. Clinica Chimica Acta, 491(January), 85–90.

Vorhees, C., & Williams, M. T. (2010). Forms of Learning and Memory. Neurology, 1(2), 848–858.

Vorhees, C. V., & Williams, M. T. (2014). Assessing spatial learning and memory in rodents. ILAR Journal, 55(2), 310–332.

Wiltgen, B. J., Zhou, M., Cai, Y., Balaji, J., Karlsson, M. G., Parivash, S. N., … Silva, A. J. (2010). The hippocampus plays a selective role in the retrieval of detailed contextual memories. Current Biology, 20(15), 1336–1344.

Article Statistic

Abstract view : 25 times
Fulltext (Bahasa Indonesia) views : 10 times

Dimensions Metrics

How To Cite This :


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.