Anti-atherogenic Effect of Mulberry Leaf Tea in Atherogenic Index on Rats Dyslipidemia

Elvi Susanti(1*), Mohammad Sulchan(2), Endang Mahati(3)
(1) Universitas Diponegoro
(2) Department of Nutrition Science, Faculty of Medicine, Diponegoro University
(3) Department of Pharmacology, Faculty of Medicine, Diponegoro University
(*) Corresponding Author
DOI : 10.30604/jika.v7i3.1013


Dyslipidemia is a lipid metabolism disorder characterized by lipid fraction changes in the blood, such as an increase in total cholesterol, LDL, TG and a decrease in HDL, which is strongly associated with atherosclerosis. Mulberry leaves (Morus alba L.) contain quercetin and 1-deoxynojirimycin (DNJ), which help to lower cardiometabolic risk by reducing hyperlipidemia, oxidative stress and atherosclerosis. The objective of this study was to determine the effect of mulberry leaf tea (Morus alba L.) on the atherogenic index (AI) in Sprague Dawley rats with dyslipidemia. This study was true experimental research with a randomized pre and post-test control group design. A total of 30 male Sprague Dawley (SD) rats were randomly divided into five groups. Calculation of AI value: log (TG/HDL-C). Statistical analysis that was used was the paired t-test and one-way ANOVA test. The intervention of mulberry leaf tea with the dosage of 36 mg/100 g BW/day (P1) and 72 mg/100 g BW/day (P2) significantly reduced atherogenic index values. Simvastatin and P2 groups showed that there was no difference in atherogenic index values after the intervention. In conclusion, giving mulberry leaf tea reduced atherogenic index values in SD rats with dyslipidemia.


Abstrak: Dislipidemia merupakan kelainan metabolisme lipid yang ditandai dengan perubahan fraksi lipid dalam darah seperti peningkatan kolesterol total, LDL, TG dan penurunan HDL yang berhubungan kuat pada kejadian aterosklerosis. Daun mulberry (Morus alba L.) mengandung kuersetin dan 1-deoxynojirimycin (DNJ) yang berguna dalam menurunkan risiko kardiometabolik dengan menurunkan keadaan hiperlipidemia, stress oksidatif dan aterosklerosis. Tujuan dari penelitian ini untuk mengetahui pengaruh teh daun mulberry (Morus alba L.) terhadap indeks aterogenik plasma (IAP) pada tikus Sprague Dawley dislipidemia. Penelitian ini merupakan penelitian true experimental dengan randomized pre and post test control group design. Sebanyak 30 ekor tikus Sprague Dawley (SD) jantan dirandomisasi menjadi 5 kelompok. Rumus perhitungan nilai IAP : log (TG/HDL-C). Analisis statistik yang digunakan adalah uji paired t-test dan uji one-way ANOVA. Intervensi teh daun mulberry dosis 36 mg/100 g BB/hari (P1) dan 72 mg/100 g BB/hari (P2) secara signifikan menurunkan nilai indeks aterogenik plasma. Pada kelompok S dan kelompok P2 menunjukkan bahwa tidak terdapat perbedaan nilai indeks aterogenik plasma setelah intervensi. Sehingga disimpulkan bahwa pemberian teh daun mulberry terbukti menurunkan nilai indeks aterogenik plasma pada tikus SD dislipidemia.


mulberry leaf tea; atherogenic index; atherosclerosis; dyslipidemia


Cho, S. K., Kim, J. W., Huh, J. H., & Lee, K. J. (2020). Atherogenic Index of Plasma Is a Potential Biomarker for Severe Acute Pancreatitis: A Prospective Observational Study. Journal of Clinical Medicine, 9(9), 2982.

Deng, Q., Li, X. X., Fang, Y., Chen, X., & Xue, J. (2020). Therapeutic Potential of Quercetin as an Antiatherosclerotic Agent in Atherosclerotic Cardiovascular Disease: A Review. Evidence-Based Complementary and Alternative Medicine, 2020, 1–12.

Dobiášová, M., Frohlich, J., Šedová, M., Cheung, M. C., & Brown, B. G. (2011). Cholesterol esterification and atherogenic index of plasma correlate with lipoprotein size and findings on coronary angiography. Journal of Lipid Research, 52(3), 566–571.

Hodel, C. (2002). Myopathy and rhabdomyolysis with lipid-lowering drugs. Toxicology Letters, 128(1–3), 159–168.

Irmayanti, L., & Ardiaria, M. (2016). Efek pemberian seduhan kulit buah naga merah (Hylocererus Polyhizus) terhadap kadar kolesterol LDL tikus sprague dawley dislipidemia. Journal of Nutrtion College, 4(5), 530–538.

James, S. R., Ray, L., Ravichandran, K., & Nanda, S. K. (2016). High atherogenic index of plasma in subclinical hypothyroidism: Implications in assessment of cardiovascular disease risk. Indian Journal of Endocrinology and Metabolism, 20(5), 656–661.

Kementrian Kesehatan RI. (2018). Laporan Riskesdas 2018. Laporan Nasional RIskesdas 2018, 53(9), 181–222. No. 57 Tahun 2013 tentang PTRM.pdf

Khazaál, M. S. (2013). Atherogenic Index of Plasma (AIP) As a Parameter in Predicting Cardiovascular Risk in Males Compared To the Conventional Dyslipidemic Indices (Cholesterol Ratios). Journal of Medicine, 6(1), 1506–1513.

Kojima, Y., Kimura, T., Nakagawa, K., Asai, A., Hasumi, K., Oikawa, S., & Miyazawa, T. (2010). Effects of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humans. Journal of Clinical Biochemistry and Nutrition, 47(2), 155–161.

Lacerda, D. D. S., de Almeida, M. G., Teixeira, C., de Jesus, A., Júnior, É. da S. P., Bock, P. M., Henriques, J. A. P., Gomez, R., Dani, C., & Funchal, C. (2018). Biochemical and physiological parameters in rats fed with high-fat diet: The protective effect of chronic treatment with purple grape juice (bordo variety). Beverages, 4(4), 1–13.

Liang, J., Fu, J., Jiang, Y., Dong, G., Wang, X., & Wu, W. (2015). TriGlycerides and high-density lipoprotein cholesterol ratio compared with homeostasis model assessment insulin resistance indexes in screening for metabolic syndrome in the chinese obese children: A cross section study. BMC Pediatrics, 15(1), 1–7.

Lim, H. H., Lee, S. O., Kim, S. Y., Yang, S. J., & Lim, Y. (2013). Anti-inflammatory and antiobesity effects of mulberry leaf and fruit extract on high fat diet-induced obesity. Experimental Biology and Medicine, 238(10), 1160–1169.

Maulana, H., & Ridwan, A. (2021). High-Fat Diets-Induced Metabolic Disorders to Study Molecular Mechanism of Hyperlipidemia in Rats. 3BIO: Journal of Biological Science, Technology and Management, 3(2), 38–50.

Metwally, F. M., Rashad, H., & Mahmoud, A. A. (2019). Morus alba L. Diminishes visceral adiposity, insulin resistance, behavioral alterations via regulation of gene expression of leptin, resistin and adiponectin in rats fed a high-cholesterol diet. Physiology and Behavior, 201, 1–11.

Niroumand, S., Khajedaluee, M., Khadem-Rezaiyan, M., Abrishami, M., Juya, M., Khodaee, G., & Dadgarmoghaddam, M. (2015). Atherogenic Index of Plasma (AIP): A marker of cardiovascular disease. Medical Journal of the Islamic Republic of Iran, 29(1), 627–635.

Nogay, N. H. (2017). Assessment of the correlation between the atherogenic index of plasma and cardiometabolic risk factors in children and adolescents: Might it be superior to the TG/HDL-C ratio? Journal of Pediatric Endocrinology and Metabolism, 30(9), 947–955.

Oikonomidis, N., Kavantzas, N., Korou, L. M., Konstantopoulos, P., Pergialiotis, V., Misiakos, E., Rizos, I., Verikokos, C., & Perrea, D. N. (2016). Pre-treatment with simvastatin prevents the induction of diet-induced atherosclerosis in a rabbit model. Biomedical Reports, 5(6), 667–674.

Paumelle, R., & Staels, B. (2007). Peroxisome proliferator-activated receptors mediate pleiotropic actions of statins. Circulation Research, 100(10), 1394–1395.

Przygo?ski, K., & Wojtowicz, E. (2019). The optimization of extraction process of white mulberry leaves and the characteristic bioactive properties its powder extract. Herba Polonica, 65(1), 12–19.

Restuti, A. N. S., & Yulianti, A. (2019). Modification of high fat diet and cattle brain sonde to weight changes in wistar. The Second International Conference on Food and Agriculture, 2, 70–73.

Rodrigues, E. L., Marcelino, G., Silva, G. T., Figueiredo, P. S., Garcez, W. S., Corsino, J., Guimarães, R. de C. A., & Freitas, K. de C. (2019). Nutraceutical and medicinal potential of the Morus species in metabolic dysfunctions. International Journal of Molecular Sciences, 20(2).

Sa’Adah, N. N., Purwani, K. I., Nurhayati, A. P. D., & Ashuri, N. M. (2017). Analysis of lipid profile and atherogenic index in hyperlipidemic rat (Rattus norvegicus Berkenhout, 1769) that given the methanolic extract of Parijoto (Medinilla speciosa). AIP Conference Proceedings, 1854(2017).

Schonewille, M., De Boer, J. F., Mele, L., Wolters, H., Bloks, V. W., Wolters, J. C., Kuivenhoven, J. A., Tietge, U. J. F., Brufau, G., & Groen, A. K. (2016). Statins increase hepatic cholesterol synthesis and stimulate fecal cholesterol elimination in mice. Journal of Lipid Research, 57(8), 1455–1464.

Shi, J., Li, R., Liu, Y., Lu, H., Yu, L., & Zhang, F. (2019). Hypercholesterolemia through the Reduction of Cholesterol Synthesis in Rat Fed a High Cholesterol Diet. Journal Hindawi Biomed Research International, 2019, 1–12.

Singh, P., Zhang, Y., Sharma, P., Covassin, N., Soucek, F., Friedman, P. A., & Somers, V. K. (2018). Statins decrease leptin expression in human white adipocytes. Physiological Reports, 6(2), 1–8.

Srinivasan, S., & Pari, L. (2013). Antihyperlipidemic effect of diosmin: A citrus flavonoid on lipid metabolism in experimental diabetic rats. Journal of Functional Foods, 5(1), 484–492.

Tenda, P. E., & Toyo, E. M. (2021). Anti-Cholesterolemic Activity of Mulberry (Morus australis Poir) Ethanol Extract in Increasing HDL Levels and Inhibiting Formation of Foam Cells on Rat. Jurnal Info Kesehatan, 19(1), 77–84.

Thaipitakwong, T., Numhom, S., & Aramwit, P. (2018). Mulberry leaves and their potential effects against cardiometabolic risks: A review of chemical compositions, biological properties and clinical efficacy. Pharmaceutical Biology, 56(1), 109–118.

Tond, S. B., Fallah, S., Salemi, Z., & Seifi, M. (2016). Influence of mulberry leaf extract on serum adiponectin, visfatin and lipid profile levels in type 2 diabetic rats. Brazilian Archives of Biology and Technology, 59(December), 1–9.

Wang, Liang, Wang, H., Zhang, B., Popkin, B. M., & Du, S. (2020). Elevated fat intake increases body weight and the risk of overweight and obesity among chinese adults: 1991–2015 trends. Nutrients, 12(11), 1–13.

Wang, Luzhao, Chen, F., Xiaoqi, C., Yujun, C., & Zijie, L. (2020). Atherogenic Index of Plasma Is an Independent Risk Factor for Coronary Artery Disease and a Higher SYNTAX Score. Angiology, 1–6.

Ward, N. C., Watts, G. F., & Eckel, R. H. (2019). Statin Toxicity: Mechanistic Insights and Clinical Implications. Circulation Research, 124(2), 328–350.

Wilson, R. D., & Islam, M. D. S. (2015). Effects of white mulberry (Morus alba) leaf tea investigated in a type 2 diabetes model of rats. Acta Poloniae Pharmaceutica - Drug Research, 72(1), 153–160.

Yi, H., Peng, H., Wu, X., Xu, X., Kuang, T., Zhang, J., Du, L., & Fan, G. (2021). The Therapeutic Effects and Mechanisms of Quercetin on Metabolic Diseases: Pharmacological Data and Clinical Evidence. Oxidative Medicine and Cellular Longevity, 2021.

Yuniarti, C. A., Sri, R., Rahayu, R., & Yuniastuti, A. (2019). Hypocholesterolemic Effect of Beet Root Extract (beta vulgaris) in Rats. Public Health Perspective Journal, 4(3), 214–223.

Zhang, Q., Fan, X., Ye, R., Hu, Y., Zheng, T., Shi, R., Cheng, W., Lv, X., Chen, L., & Liang, P. (2020). The Effect of Simvastatin on Gut Microbiota and Lipid Metabolism in Hyperlipidemic Rats Induced by a High-Fat Diet. Frontiers in Pharmacology, 11.

Zhong, L., Furne, J. K., & Levitt, M. D. (2006). An extract of black, green, and mulberry teas causes malabsorption of carbohydrate but not of triacylglycerol in healthy volunteers. American Journal of Clinical Nutrition, 84(3), 551–555.

Zhukova, N. V., Novgorodtseva, T. P., & Denisenko, Y. K. (2014). Effect of the prolonged high-fat diet on the fatty acid metabolism in rat blood and liver. Lipids in Health and Disease, 13(1), 1–8.

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