Effectiveness of Pumpkin Extract (Cucurbita moschata) Lowering Blood Glucose and Malondialdehyde Levels in Diabetic Model Rats

Ma'rifatul Khoiriyah Ayda(1*), Muthmainah Muthmainah(2), Ratih Puspita Febrinasari(3)
(1) Universitas Sebelas Maret
(2) Department of Histology, Faculty of Medicine, Sebelas Maret University
(3) Departement of Pharmacology, Faculty of Medicine, Sebelas Maret University
(*) Corresponding Author
DOI : 10.30604/jika.v7i4.1401

Abstract

Hyperglycemia in uncontrolled diabetes mellitus (DM) can cause oxidative stress, among others, characterized by high levels of malondialdehyde (MDA). This situation continues to cause various complications. Pumpkin (Cucurbita moschata) is known to contain various antioxidants that can reduce blood glucose levels and oxidative stress levels in DM. This study aims to prove the effectiveness of pumpkin in the form of an extract to reduce fasting blood glucose (FBG)and MDA levels in DM rats.The study used 36 male Wistar rats which were divided into 6 groups, namely:KN:normal group;K(-):DM group;K(+):DM group was given glibenclamide 0.45 mg/kgBW/day;P1,P2,P3:DM group which was given the extract with a successive dose of 0.66mg/kgBW/day,1.32mg/200gBW/day,and 2.64mg/200gBW/day.The extract was administered for 21 consecutive days. The DM model was made by induction of streptozotocin (STZ) and nicotinamide (NA). FBG and MDA levels were measured serially 5 times and analyzed statistically. The results showed that the higher the dose of the extract and the longer the administration of the extract, the more effective the extract's ability to reduce blood FBG and MDA levels. Compared to glibenclamide, pumpkin extract was no less effective in reducing blood FBG and MDA levels. It was concluded that pumpkin extract was effective in lowering blood glucose and MDA levels in DM rats.

 

Abstrak: Hiperglikemia pada diabetes melitus (DM) yang tidak terkontrol dapat menyebabkan stres oksidatif antara lain ditandai dengan tingginya kadar malondialdehid (MDA). Keadaan ini terus menimbulkan berbagai komplikasi. Labu kuning (Cucurbita moschata) diketahui mengandung berbagai antioksidan yang dapat menurunkan kadar glukosa darah dan tingkat stres oksidatif pada DM. Penelitian ini bertujuan untuk membuktikan efektivitas labu kuning dalam bentuk ekstrak untuk menurunkan kadar glukosa darah puasa (GDP) dan MDA pada tikus DM. Penelitian menggunakan 36 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok, yaitu: KN: kelompok normal; K(-): grup DM; K(+): Kelompok DM diberikan glibenklamid 0,45 mg/kgBB/hari; P1, P2, P3 : Kelompok DM yang diberi ekstrak dengan dosis berturut-turut 0.66mg/kgBB/hari, 1.32mg/200gBB/hari dan 2.64mg/200gBB/hari. Ekstrak diberikan selama 21 hari berturut-turut. Model DM dibuat dengan induksi streptozotocin (STZ) dan nicotinamide (NA). Tingkat GDP dan MDA diukur secara serial sebanyak 5 kali dan dianalisis secara statistik. Hasil penelitian menunjukkan bahwa semakin tinggi dosis ekstrak dan semakin lama pemberian ekstrak, semakin efektif kemampuan ekstrak dalam menurunkan kadar GDP dan MDA darah. Dibandingkan dengan glibenclamide, ekstrak labu tidak kalah efektif dalam menurunkan kadar GDP dan MDA darah. Disimpulkan bahwa ekstrak labu kuning efektif menurunkan kadar glukosa darah dan MDA pada tikus DM.

Keywords


pumpkin extract; blood glucose; malondialdehyde; diabetes mellitus

References


Akbari, A., Jelodar, G., Nazifi, S., & Sajedianfard, J. (2016). An Overview of the Characteristics and Function of Vitamin C in Various Tissues : Relying on its Antioxidant Function. 18(11). https://doi.org/10.17795/zjrms-4037.Review

Alam, S., Khan, Z. I., Mustafa, G., Kumar, M., Islam, F., Bhatnagar, A., & Ahmad, F. J. (2012). Development and evaluation of thymoquinone-encapsulated chitosan nanoparticles for nose-to-brain targeting: A pharmacoscintigraphic study. International Journal of Nanomedicine, 7, 5705–5718. https://doi.org/10.2147/IJN.S35329

Arifin, W. N., & Zahiruddin, W. M. (2017). Sample size calculation in animal studies using resource equation approach. Malaysian Journal of Medical Sciences, 24(5), 101–105. https://doi.org/10.21315/mjms2017.24.5.11

Bowser Riley, F., & House, C. R. (2021). The actions of some putative neurotransmitters on the cockroach salivary gland. In Journal of Experimental Biology (Vol. 64, Issue 3). https://doi.org/10.1242/jeb.64.3.665

Decroli. (2019). Buku Diabetes Melitus Tipe 2. Pusat Penerbitan Bagian Ilmu Penyakit Dalam. http://repo.unand.ac.id/21867/1/Buku Diabetes Melitus %28Lengkap%29.pdf

Díaz-Flores, M., Angeles-Mejia, S., Baiza-Gutman, L. A., Medina-Navarro, R., Hernández-Saavedra, D., Ortega-Camarillo, C., Roman-Ramos, R., Cruz, M., & Alarcon-Aguilar, F. J. (2012). Effect of an aqueous extract of Cucurbita ficifolia Bouché on the glutathione redox cycle in mice with STZ-induced diabetes. Journal of Ethnopharmacology, 144(1), 101–108. https://doi.org/10.1016/j.jep.2012.08.036

Dini, C. Y. (2015). Pengaruh Tepung Labu Kuning Terhadap Ekspresi Srebp- Dyslipidemia Effect Of Pumpkin Powder On Hepatic And White Adipose Tissue Srebp-1c Expression In Dislipidemia Rats Model. Thesis, 0–1. http://etd.repository.ugm.ac.id/penelitian/detail/88381

Fajrilah, B. (2016). Pengaruh Pemberian Madu Terhadap Kadar Malondialdehyde (Mda) Plasma Darah Pada Tikus Yang Diinduksi Alloxan - Studi Experimental Pada Tikus Putih Jantan Galur Wistar. Skripsi, https://news.ge/anakliis-porti-aris-qveynis-momava.

Faqih, A. (2017). Efek Ekstrak Buah Labu Kuning (Cucurbita Moschata) Terhadap Penurunan Kadar Glukosa Darah Postprandial Pada Tikus Putih ( Rattus Norvegicus ) Abstrak Effect Of Extract Yellow Pumpkin Fruit ( Cucurbita Moschata ) To Decrease Of Postprandial Blood Glucose. Skripsi, 002. https://repository.upnvj.ac.id/4393/

Fathonah, R., Indriyanti, A., & Kharisma, Y. (2014). Labu Kuning (Cucurbita moschata Durch.) untuk Penurunan Kadar Glukosa Darah Puasa pada Tikus Model Diabetik. In Global Medical & Health Communication (Vol. 2, Issue 1, pp. 27–33). https://ejournal.unisba.ac.id/index.php/gmhc/article/view/1527

Ghasemi, A., Khalifi, S., & Jedi, S. (2014). Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiologica Hungarica, 101(4), 408–420. https://doi.org/10.1556/APhysiol.101.2014.4.2

Hanum, N. N. (2013). Hubungan Kadar Glukosa Darah Puasa dengan Profil Lipid pada Pasien Diabetes Melitus tipe 2 di Rumah Sakit Umum Daerah Cilegon periode Januari-April 2013. Program Studi Pendidikan Dokter Fakultas Kedokteran Dan Ilmu Kesehatan Universitas Islam Negeri Syarif Hidaytullah, April, 1–70. https://repository.uinjkt.ac.id/dspace/bitstream/123456789/26407/1/Nida Najibah Hanum-FKIK.pdf

Kamilatussaniah, Yuniasti, A., & Iswari, R. (2015). Pengaruh Suplementasi Madu Kelengkeng Terhadap Kadar Tsa Dan Mda Tikus Putih Yang Diinduksi Timbal (Pb). Jurnal MIPA Unnes, 38(2), 108–114. https://journal.unnes.ac.id/nju/index.php/JM/article/view/5779

Karuranga, et al. (2019). International Diabetes Federation. In The Lancet (Vol. 266, Issue 6881). https://doi.org/10.1016/S0140-6736(55)92135-8

Kemenkes RI. (2020). Tetap Produktif, Cegah Dan Atasi Diabetes Mellitus. In pusat data dan informasi kementrian kesehatan RI.

Latumahina, G. J. (2011). Peran Madu Sebagai Antioksidan Dalam Mencegah Kerusakan Pankreas Mencit (Mus Musculus) Terpapar Asap Rokok Kretek. Jurnal Kedokteran Dan Kesehatan Program Studi Pendidikan Dokter Universitas Pattimura, 106. http://ejournal.unpatti.ac.id/ppr_iteminfo_lnk.php?id=578

Lismawati, Tutik, & Nofita. (2021). Kandungan Beta Karoten Dan Aktivitas Antioksidan Terhadap Ekstrak Buah Labu Kuning (Cucurbita moschata). Jurnal Mandala Pharmacon Indonesia, 7(2), 263–273. http://jurnal-pharmaconmw.com/jmpi/index.php/jmpi/article/view/111

Loscalzo, D. E. H. R. C. J. (2011). NIH Public Access. Bone, 23(1), 1–7. https://doi.org/10.1016/j.jnutbio.2013.06.003.Recent

Monteiro. (2014). Pengaruh Pemberian Ekstrak Labu Kuning Per Oral (Cucurbita Moschata Duchenes) Terhadap Kadar Trigliserida Tikus Jantan (Rattus Norvegicus Strain Wistar) Model Diabetes Mellitus Tipe 2. Paper Knowledge . Toward a Media History of Documents, 7(2), 107–115. http://repository.ub.ac.id/id/eprint/123489/

Nahwa, A. (2008). Kadar malondialdehyde (MDA) serum sebagai indikator porgnisis pada sepsis neonatorum. Thesis. http://eprints.undip.ac.id/18343/

Novianti Muhammad; Widowati, Ita, T. Z. (2019). Aktivitas Antioksidan dan Identifikasi Golongan Senyawa Aktif Ekstrak Kasar Mikroalga Chlorella Vulgaris Yang Dikultivasi Berdasarkan Sumber Cahaya Yang Berbeda. Barakuda 45: Jurnal Ilmu Perikanan Dan Kelautan, Vol 1 No 2 (2019): Edisi November, 72–87. https://doi.org/10.47685/barakuda45.v1i2.44

Ortiz-Andrade, R. R., Sánchez-Salgado, J. C., Navarrete-Vázquez, G., Webster, S. P., Binnie, M., García-Jiménez, S., León-Rivera, I., Cigarroa-Vázquez, P., Villalobos-Molina, R., & Estrada-Soto, S. (2008). Antidiabetic and toxicological evaluations of naringenin in normoglycaemic and NIDDM rat models and its implications on extra-pancreatic glucose regulation. Diabetes, Obesity and Metabolism, 10(11), 1097–1104. https://doi.org/10.1111/j.1463-1326.2008.00869.x

Pathy. (2018). Process for Preparation of Vitamin C and Method for Determination of Vitamin C in Tablets. 1(3), 52–65. https://doi.org/10.32474/SCSOAJ.2018.01.000114

Rahayu, M., Kasiyati, M., Martsiningsih, A., Setiawan, B., & Khasanah, F. (2020). Hypoglicemic and Antioxidant Activity of Yellow Pumpkin (Curcubitamoschata) in Diabetic Rats. Indian Journal of Public Health Research & Development, 11(1), 1300. https://doi.org/10.37506/v11/i1/2020/ijphrd/194022

Rusiani, E., Junaidi, S., Subiyono, H. S., & Sumartiningsih, S. (2019). Suplementasi Vitamin C dan E untuk Menurunkan Stres Oksidatif Setelah Melakukan Aktivitas Fisik Maksimal. 9. https://journal.unnes.ac.id/nju/index.php/miki/article/view/23582

Sayuti, K., & Yenrina, R. (2015). Alami dan Sintetik (1 ed). https://adoc.pub/antioksidan-alami-dan-sintetik.html

Setiawan. (2016). Efek Antihiperglikemik Buah Labu Kuning (Cucurbita moschata duch) terhadap kadar glukosa darah, ekspresi insulin, dan profil lipoprotein tikus putih (Rattus Novergicus L) hiperglikemik o Title No Title No Title. 15(2), 1–23.

Subiyono, Martsiningsih, M. A., & Gabrela, D. (2016). Gambaran kadar glukosa darah metode GOD-PAP (Glucose Oxsidase – Peroxidase Aminoantypirin) sampel serum dan plasma EDTA (Ethylen Diamin Terta Acetat). Jurnal Teknologi Laboratorium, 5(1), 45–48. https://www.teknolabjournal.com/index.php/Jtl/article/view/77

Suwanto, S., Pratiko Gustomi, M., & Kurnijasanti, R. (2020). Potensi Ekstrak Etanol Biji Cucurbita moschata terhadap Kadar Malondehaldehid Mencit Model Diabetes. Indonesian Journal of Human Nutrition, 7(1), 20–30. https://doi.org/10.21776/ub.ijhn.2020.007.01.3

Szkudelski, T., Zywert, A., & Szkudelska, K. (2013). Metabolic disturbances and defects in insulin secretion in rats with streptozotocin-nicotinamide-induced diabetes. Physiological Research, 62(6), 663–670. https://doi.org/10.33549/physiolres.932509

Werdhasari, A. (2014). Peran Antioksidan Bagi Kesehatan. Jurnal Biomedik Medisiana Indonesia, 3(2), 59–68. http://ejournal.litbang.kemkes.go.id/index.php/jbmi/article/view/4203

Widowati. (2008). Potensi Antioksidan sebagai Antidiabetes. JKM, 7(2), 1–11. https://www.neliti.com/id/publications/149640/potensi-antioksidan-sebagai-antidiabetes

Widowati, S. (2009). Tepung Aneka Umbi Sebuah Solusi Ketahanan Pangan. Jurnal Sinar Tani, 6–12. http://old.litbang.pertanian.go.id/artikel/one/240/pdf/Tepung Aneka Umbi Sebuah Solusi Ketahanan Pangan.pdf


Article Statistic

Abstract view : 135 times
PDF (Bahasa Indonesia) views : 23 times

Dimensions Metrics

How To Cite This :

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Ma'rifatul Khoiriyah Ayda, Muthmainah Muthmainah, Ratih Puspita Febrinasari

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.