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Testing the larvacides activities of clove leaves extract (Syzygium aromaticum) on the mortality of Aedes Aegypti L.

Ariyani Buang(1*), Farid Fani Temarwut(2), Suprapto Prayitno(3)
(1) Universitas Pancasakti
(2) Universitas Pancasakti
(3) Universitas Pancasakti
(*) Corresponding Author
DOI : 10.30604/jika.v8i2.2048

Abstract

Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus which is transmitted by the Aedes Aegypti mosquito. Clove leaves (Syzigum aromaticum) is a part of a plant that contains eugenol, saponins, flavonoids and tannins which are useful as natural larvicides against Aedes Aegypti mosquito larvae.This study aims to determine the effect of larvicides Clove leaf extract (Syzigium aromaticum) on Aedes aegypti L larvae and to determine the LC50 value that can kill Aedes aegypti L larvae. This study was an experimental study with a post test only group control design. Clove leaves were extracted by maceration using 96% w/v ethanol.This study was divided into 7 groups using Aedes aegypty Instar III larvae, namely:2 control groups namely K1 NaCMC 1% as negative control and K6 Abate 0.1% b/v as positive controland 5 treatment groups each with a concentration of clove leaf extract K20.010 % w/v, K3 0.025 % w/v, K4 0.05 % b/v, K5 0.075 % b/v and K6 0.100 % b/v.The number of larvae deaths was counted after 24 hours.HThe results of the study showed that the number of deaths of Aedes aegypti mosquito larvae for each group was: K1 0%, K2 3.33%, K3 8.33%, K4 23.33%, K5 53.33%, K6 80% and K7 100 %Analysis results statistics using Anova. and the LSD follow-up test isthere was a significant difference in larval mortality at each concentration (p 0.00 less than 0.05) And K2 showed a non-significant effect with K3 (sig p 0.089 more than 0.05). From the research results it can be concluded that clove leaf extract (Syzygium aromaticum L) has a larvicidal effect on Aedes aegypti larvae. Clove leaf extract (Syzygium aromaticum) can kill Aedes aegypti mosquito larvae with an LC50 value of 600 ppm and is included in the toxic category.

Keywords


Clove leaf (Szigium aromaticum); Extract, Aedes aegypti larvae; LC50

References


Adrianto.H dkk 2022. Entomologi Untuk Kedokteran dan Kesehatan. Rapha Publishing

Arivia, S., Kurniawan, B., Zuraida, R. (2013). Efek larvasida ekstrak daun lidah buaya (Aloe vera) terhadap larva aedes aegypti instar III. Medical Journal of Lampung University.

Ansel,H.C (1989). Pengantar bentuk Sediaan Farmasi, Penerjemah : Farida Ibrahim.2008.UI Press. Jakarta.

Chintithia, T., 2015, Efek Larvasida Ekstrak Daun Cengkeh (Syzygium aromaticm) terhadap Larva Aedes egypti. Fakultas Kedokteran, Universitas Lampung.

Cabia, E dan Setyaningrum, E (2012) Larvacide Effectiveness Test of the Legundi’s Leaf (Vitex trifolia) Extraxt for Larvae of Aedes aegypti. Lampung : Medical Faculty of Lampung University.

Departemen Kesehatan Republik Indonesia. (2004) Kebijaksanaan Program P2 DBD dan Situasi Terkini DBD di Indonesia. diakses pada tanggal 27 Mei 2022.

Depkes. RI.2006. Parameter Standar Umum Ekstrak Tumbuhan Obat. Depkes RI. Jakarta

Dewi,C.I.D.Y, Ernawati,D.K, Widhiartini,I.A.A. (2021). Uji Efektivitas Ekstrak Etanol Daun Cengkeh (Syzygium aromaticum L.) Terhadap Pertumbuhan Methicillin Resistant Staphylococcus aureus Secara In Vitro. Jurnal Medika Udayana.Vol.10. No.2.

Fuadzy, H., Hodijah, D.N., Jajang, A & Widawati, M (2015) Kerentanan larva aedes aegypti terhadap termefos di tiga kelurahan endemis demam berdarah dengue Kota Sukabumi. Buletin Penelitian Kesehatan, 43(1)

Fatmawati,S. (2019). Pengaruh Perbedaan Metode Ekstraksi Perkolasi dan Maserasi Terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Naga Merah Hylozereus polyrhizus. Politeknik Harapan Bersama Kota Tegal.

Fayemiwo, K. A., Adeleke, M. A., Okoro, O. P., Awojide, S. H., Olufemi, I., Awoniyi. (2014). Larvicidal efficacies and chemical composition of essential oils of Pinus sylvestris and Syzygium aromaticum against mosquitos. Asian Pacific Journal of Tropical Biomedicine. 4(1)

Gautar, Kumar dan Poonia.(2013). Larvacidal activity and GC-MS analysis of Flavonoid of Vitex negundo and Andrographis paniculata againts two vector mosquitoes Anopheles stephensi and Aedes aegyptiJ Vector Borne 50 (9).

Haditomo,I (2010).Efek Larvasida Ekstrak Daun Cengkeh (Syzygium aromaticum L) Terhadap Aedes Aegypti L.Skripsi. Fakultas Kedokteran Universitas Sebelas Maret Surakarta.

Harborne, J.B. (1996). Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan. Penerjemah. K. Padmawinata dan L.Sudiro. Terbitan Ke-12. ITB Bandung.

Kementerian Kesehatan RI. (2022) Profil Kesehatan Indonesia Tahun 2021. .Kementerian Kesehatan Republik Indonesia.Jakarta.

Kardinan A. (2003). Tanaman pengusir danpembasmi nyamuk. Jakarta: AgroMedia Pustaka.

Kusumawati,W.D; Subagiyo,A; Firdaus,M. (2018). Pengaruh Beberapa Dosis dan Jenis Ekstrak Larvasida Alami Terhadap Kematian Larva Nyamuk Aedes aegypti. Politeknik Kesehatan Kemenkes Semarang.

Lambiju, E.M, Wowor, P.M, Leman, M.A. (2017) Uji Daya Hambat Ekstrak Daun Cengkeh (Syzygium aromaticum) Terhadap Bakteri Enterococcus faecalis. JurnalMe-GiGi.

Marjodi, Mhd, (2016). Dasar dasar Fitokimia untuk Diploma III Farmasi. 1 ed. Jakarta. Trans Info Media.

Mulyatno KC, Yamanaka A, Ngadino, Konishi E (2012) Resistance of Aedes Aegypti (L) Larvae to temefos in Surabaya, Indonesia. Southeast Asian J Trop Med Public Health (43).

Marcombe, Sebastien et all (2011). Field Efficacy of New Larvacide Product for Control of Multiresistant Aedes aegypti Population in Martinique (French West Indies). The American Society of Tropical Medicine and Hygiene,84(1):

Megha,N.M dan Sabale,A.B.(2014). Antimicrobial, Antioxidant and Haemolityc Potential of Brown Marcoalga Sargosum. World Journal of Pharmacy and Pharmaceurical Sciences. 3(*).

Natadisastra,D dan Agoes,R.2009. Parasitologi Kedokteran Ditinjau dari Organ Tubuh yang Diserang.EGC.

NCBI (2015). Aedes aegypti Culturing and Eggs Colletion

Pratiwi, A. (2014). Studi deskkriptif penerimaan masyarakat terhadap larvasida alami. Jurnal Ilmu Kesehatan Masyarakat, 3(2)

Prihatna,K. (2001). Saponin Untuk Pembasmi Hama Udang. Penelitian Perkebunan Gambung.Bandung.

Riwanti,P., Iszazih, F & Amaliyah,A. 2020. Pengaruh perbedaan Konsentrasi Etanol 50%, 70% dan 96% Pada Kadar Flavonoid Total Ekstrak Etanol Sargassum polycystum Dari Madura. Journal of Pharmaceutical Care Anwar Medika.

Suwasono H.(1997). Berbagai Cara Pemberantasan Larva Aedes aegypti, Cermin Dunia Kedokteran.

Septiani, Nurcahya, E. D., Wijayanti, I. (2017) Aktivitas Antibakteri Ekstrak Lamun Terhadap Bakteri AktivitasMAntibakteri EkstrakMLamun (Cymodocea Rotundata) Terhadap Bakteri Staphylococcusnaureus dan Escherichia coli. Indonesian Journal of Fisheries Sciences and Technology.

Santoso,J.,Anwariyah,S., Rumiantim,R.O., Putri,A,P., Ukhty,N dan Yoshie-stark,Y (2012). Phenol Content Antioxidant Activity and Fibers Profile of Four Tropical Seagrasses From Indonesia. Journal of Coastal Development, 15(2).

SutthanontN, Choochote W, Tuetun B, Junkum A, JitpakdA, Chaithong U, Riyong D, Pitasawat, B.(2010). Chemical composition and larvicidal activity of edible plant-derived essential oils against the pyrethroidsusceptible and resistant strains of Aedes aegypti (Diptera: Culicidae). Journal of Vector Ecology. 35 (1): 1

Torres,S.M dkk (2014). Cululative Mortality of Aedes aegypti Larvae Treated with Compounds. Rev Saude Publicia. 48(3).

Tandi,E.,J.2010. Pengaruh Tanin Terhadap Aktivitas Enzim Protease. Seminar Nasional Teknologi Peternakan dan Veteriner. Fakultas Peternakan UNHAS.

Yasi, RM, dan Harsanti, RS, 2018, Pengaruh Variasi Konsentrasi Ekstrak Daun Kelor (Moringa aloifera) terhadap Kematian Larva Aedes aegypit, Seminar Nasional Program Studi Agribisnis Fakultas Pertanian Universitas Jember, diakses tanggal 27 Oktober 2022, https://jurnal.unej.ac.id

William, D.F.(1981). Extraction With Supercritical Gases, Chem.Engineering Sci 36 (11).

WHO (2005). Guidelines for Laboratory and Field Testing of Mosquito Larvicid


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