Metabolic Syndrome Induced by Hipercalory Diet in Wistar Rats: impact on diabetic risk, dyslipidemia and hypertension complication
Abstract
Objective: Metabolic syndrome is a group of metabolic disorders that are usually found in individuals with a high risk of type 2 diabetes mellitus (T2DM) and cardiovascular disease. Comparison of diet composition, especially macronutrients, over a certainperiode in both humans and experimental animals can allegedly increase the risk of metabolic syndrome. This study aims to determine the use of a hypercaloric diet that can cause metabolic syndrome in male Wistar rats. Methods: The hypercaloric diet was administered orally in pellets and oral solution to rats for 16 weeks. Analysis of the condition of the metabolic syndrome was carried out by looking at the increase in serum levels of total cholesterol, triglycerides and decreased HDL levels. Fasting blood sugar and fasting insulin levels were also seen to increase and blood pressure testing was carried out to see an increase in blood pressure after 16 weeks of giving a hypercaloric diet. Results: Administration of a hypercaloric diet showed an increased risk of metabolic syndrome in rats. Administration for 16 weeks showed a significant difference in the improvementofs conditions of dyslipidemia, insulin resistance and hypertension. Conclusion: Based on the results, a hypercaloric diet for 16 weeks can cause metabolic syndrome in rats.
Abstrak: Objektif: Sindrom metabolik adalah sekelompok kelainan metabolik yang biasanya ditemukan pada individu dengan risiko tinggi diabetes militus tipe 2 (T2DM) dan penyakit kardiovaskular. Perbandingan komposisi diet khususnya makronutrien, dalam jangka waktu tertentu baik pada manusia maupun hewan coba disinyalir dapat meningkatkan resiko dari sindroma metabolik. Penelitian ini bertujuan untuk menentukan penggunaan diet hipercalori yang dapat menyebabkan kondisi sindrome metabolikpada tikus Wistar jantan. Metode:diet hiperkalori diberikan secara oral dalam bentuk pelet dan larutan oral pada tikus selama 16 minggu. Analisis terhadap kondisi syndrome metabolic dilakukan dengan melihat peningkatan kadar serum kolesterol total, trigliserida dan penurunan kadar HDL. Kadar gula darah puasa dan insulin puasa juga dilihat peningkatannya serta dilakukan pengujian tekanan darah untuk melihat terjadinya peningkatan tekanan darah setelah 16 minggu pemberian diet hiperkalori. Hasil: Pemberian diet hiperkalori menunjukkan adanya peningkatan risiko sindrom metabolic pada tikus. Pemberian selama 16 minggu menujukkan adanya perbedaan signifikan pada peningkatan kearah kondisi dyslipidemia, resistensi insulin dan hipertensi. Kesimpulan: Berdasarkan hasil, pemberian diet hiperkalori selama 16 minggu dapat menyebabkan terjadinya kondisi sindrom metabolic pada tikus
Keywords
References
Abdulla, M. H., Sattar, M. A., & Johns, E. J. (2011). The Relation between Fructose-Induced Metabolic Syndrome and Altered Renal Haemodynamic and Excretory Function in the Rat. International Journal of Nephrology, 2011, 1–17. https://doi.org/10.4061/2011/934659
Al-Attar, A. M. (2010). Physiological and Biochemical Alterations in Sprague-Dawley Female Rats Subjected to High Fat Diet and Intermittent Fasting. Journal of Applied Sciences Research, 6(12), 2096–2104.
Aydin, S., Aksoy, A., Aydin, S., Kalayci, M., Yilmaz, M., Kuloglu, T., … Catak, Z. (2014). Today’s and yesterday’s of pathophysiology: Biochemistry of metabolic syndrome and animal models. Nutrition, 30(1), 1–9. https://doi.org/10.1016/j.nut.2013.05.013
Aziz, A. A., Kenney, L. S., Goulet, B., & Abdel-Aal, E. S. (2009). Dietary starch type affects body weight and glycemic control in freely fed but not energy-restricted obese rats. Journal of Nutrition, 139(10), 1881–1889. https://doi.org/10.3945/jn.109.110650
Bray, G. A. (2007). How bad is fructose? American Journal of Clinical Nutrition, 86(4), 895–896. https://doi.org/10.1093/ajcn/86.4.895
Brown, M. A., Storlien, L. H., Huang, X.-F., Tapsell, L. C., Else, P. L., Higgins, J. A., & Brown, I. L. (2010). Dietary Fat and Carbohydrate Composition: Metabolic Disease. Fat Detection: Taste, Texture, and Post Ingestive Effects, (2007), 1–23. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21452465
Daniels, A., Linz, D., Van Bilsen, M., Rütten, H., Sadowski, T., Ruf, S., … Van Nieuwenhoven, F. A. (2012). Long-term severe diabetes only leads to mild cardiac diastolic dysfunction in Zucker diabetic fatty rats. European Journal of Heart Failure, 14(2), 193–201. https://doi.org/10.1093/eurjhf/hfr166
Hidayaturrahmah, Budi Santoso, H., Aulia Rahmi, R., & Kartikasari, D. (2020). Blood glucose level of white rats ( Rattus norvegicus ) after giving catfish biscuit ( Pangasius hypothalmus ) . BIO Web of Conferences, 20, 04005. https://doi.org/10.1051/bioconf/20202004005
Ihedioha, J. I., Noel-Uneke, O. A., & Ihedioha, T. E. (2013). Reference values for the serum lipid profile of albino rats (Rattus norvegicus) of varied ages and sexes. Comparative Clinical Pathology, 22(1), 93–99. https://doi.org/10.1007/s00580-011-1372-7
Lehnen, A. M., Rodrigues, B., Irigoyen, M. C., De Angelis, K., & Schaan, B. D. A. (2013). Cardiovascular changes in animal models of metabolic syndrome. Journal of Diabetes Research, 2013. https://doi.org/10.1155/2013/761314
Luo, Y., Owens, D., Mulder, G., Mcvey, A., Charles, T. F., & Laboratories, R. (n.d.). Characterization_of_Hypertensive_and_Cardiovascular_Studies.
Maryani, P. E., Ulfa, E. U., & Rachmawati, E. (2016). Pengaruh Ekstrak Metanol Daun kayu Kuning (Arcangelisia flava L. Merr) terhadap Kadar kolesterol Total dan Trigliserida Tikus Hiperlipidemia. E-Jurnal Pustaka Kesehatan, 4(1), 20–26.
Mohamed, F. A., & Bahgat, N. M. (2009). The Egyptian Journal of Hospital Medicine ( 2009 ) Vol ., 37 : 630-643 Effect of Coconut Oil Administration on Some Hemostatic Changes Associated with Obesity in Rats Effect of Coconut Oil Administration on Some … Materials and Methods : 630–643.
Nascimento, A. F., Sugizaki, M. M., Leopoldo, A. S., Lima-Leopoldo, A. P., Luvizotto, R. A. M., Nogueira, C. R., & Cicogna, A. C. (2008). A hypercaloric pellet-diet cycle induces obesity and co-morbidities in wistar rats. Arquivos Brasileiros de Endocrinologia e Metabologia, 52(6), 968–974. https://doi.org/10.1590/s0004-27302008000600007
Nielsen, S., & Karpe, F. (2012). Determinants of VLDL-triglycerides production. Current Opinion in Lipidology, 23(4), 321–326. https://doi.org/10.1097/MOL.0b013e3283544956
Panchal, S. K., Poudyal, H., Iyer, A., Nazer, R., Alam, A., Diwan, V., … Brown, L. (2011). High-carbohydrate high-fat diet-induced metabolic syndrome and cardiovascular remodeling in rats. Journal of Cardiovascular Pharmacology, 57(1), 51–64. https://doi.org/10.1097/FJC.0b013e3181feb90a
Roberts, C. K., Hevener, A., & Barnard, R. (2014). and Modification by Exercise Training. Compr Physiol, 3(1), 1–58. https://doi.org/10.1002/cphy.c110062.Metabolic
Ruslin, Kasmawati, H., Suryani, Ihsan, S., & Sartina, D. (2019). Activity Assay of Etanol Extract of Lansau as Antihyperlipidemic Ruslin Ruslin *, Henny Kasmawati , Suryani Suryani , Sunandar Ihsan , Desi Sartina Uji Aktivitas Ektrak Etanol Lansau sebagai Antihiperlipidemia. 6(3), 118–124.
Sánchez-Lozada, L. G., Tapia, E., Jiménez, A., Bautista, P., Cristóbal, M., Nepomuceno, T., … Franco, M. (2007). Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. American Journal of Physiology - Renal Physiology, 292(1), 423–429. https://doi.org/10.1152/ajprenal.00124.2006
Senaphan, K., Kukongviriyapan, U., Sangartit, W., Pakdeechote, P., Pannangpetch, P., Prachaney, P., … Kukongviriyapan, V. (2015). Ferulic acid alleviates changes in a rat model of metabolic syndrome induced by high-carbohydrate, high-fat diet. Nutrients, 7(8), 6446–6464. https://doi.org/10.3390/nu7085283
Sherwood, L. (2016). Human Physiology from Cell to System. USA: Cengage Learning.
Wiley, J., & Sons. (2011). Metabolic Syndrome Underlying Mechanisms and Drug Therapies.
Y, C. (2012). Encyclopedia of Exercise Medicine in Health and Disease. In Metabolism: Carbohydrate. In: Mooren FC, editor. Encyclopedia of Exercise Medicine in Health and Disease. Berlin: Springer Berlin Heidelberg; https://doi.org/10.1007/978-3-540-29807-6Article Statistic
Abstract view : 1560 timesPDF (Bahasa Indonesia) views : 563 times
Dimensions Metrics
How To Cite This :
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Email this article 
