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Antihyperglycemic effect of patikan kebo in the rat models of diabetic

Sherly Asri Widyaningrum(1*), Brian Wasita(2), Adi Magna Patriadi Nuhriawangsa(3)
(1) Universitas Sebelas Maret (UNS)
(2) Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret
(3) Departement of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret
(*) Corresponding Author
DOI : 10.30604/jika.v8i2.1735

Abstract

Diabetes mellitus is a heterogeneous metabolic disorder characterized by increased blood sugar levels due to insulin disorders. Current treatment for diabetes mellitus focuses on controlling and lowering blood glucose. However, long-term treatment for diabetes mellitus can have side effects that cause several medical problems. Medical therapy using natural ingredients as an alternative therapy is currently being considered because of its potential and minimal side effects. Patikan kebo is a natural ingredient containing the flavonoid quercetin group. However, the antihyperglycemic effect of the patikan kebo leaves is not known with certainty. This study aimed to determine the effect of patikan kebo leaf extract (EDPK) on the levels of GDP, insulin, HOMA-IR, and HOMA-B in male rats with diabetes mellitus induced by streptozotocin (STZ) and nicotinamide (NA). The design of this study was a pre-and post-test control group design. 42 male Wistar rats were divided into 7 groups: K (normal mice), KN (DMT2 mice without intervention), KP (DMT2 mice intervened with acarbose 1.8 mg/kg BW/day), P1 (DMT2 mice intervened with EDPK dose 75 mg/kg BW/day), P2 (DMT2 mice intervened with EDPK dose 150 mg/kg BW/day), and P3 (DMT2 rats intervened with EDPK dose 300 mg/kg BW/day). The intervention was given for 7 and 15 days. The data were statistically analyzed using the Two Way ANOVA test. EDPK was proven to decrease GDP and HOMA-IR levels and increase insulin and HOMA-B in the male Wistar rat model of diabetes mellitus induced by STZ+NA. EDPK with a dose of 300 mg/kg BW/day proved to have the strongest antihyperglycemic effect.

 

Abstrak: Diabetes melitus merupakan penyakit kelainan metabolisme heterogen yang ditandai dengan adanya peningkatan kadar gula darah akibat gangguan sekresi insulin. Pengobatan diabetes melitus saat ini berfokus pada pengendalian dan penurunan kadar glukosa darah. Akan tetapi lama terapi pengobatan pada diabetes melitus dapat memberikan efek samping yang menyebabkan beberapa masalah medis. Terapi pengobatan menggunakan bahan alami sebagai terapi alternatif saat ini lebih dipertimbangkan karena potensi serta minimalnya efek samping yang diberikan. Patikan kebo merupakan bahan alami dengan kandungan flavonoid golongan quercetin. Namun saat ini belum diketahui secara pasti efek antihiperglikemik dari daun patikan kebo tersebut. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun patikan kebo (EDPK) terhadap kadar GDP, insulin, HOMA-IR, dan HOMA-B pada tikus jantan diabetes melitus yang diinduksi Streptozotocin (STZ) dan Nicotinamide (NA). Rancangan penelitian ini dengan pre and post test controlled group design. 42 ekor tikus jantan wistar dibagi menjadi 7 kelompok; K (tikus normal), KN (tikus DMT2 tanpa intervensi), KP (tikus DMT2 diintervensi dengan acarbose 1,8 mg/kgBB/hari), P1 (tikus DMT2 diintervensi EDPK dosis 75mg/kgBB/hari), P2 (tikus DMT2 diintervensi EDPK dosis 150mg/kgBB/hari), P3 (tikus DMT2 diintervensi EDPK dosis 300mg/kgBB/hari). Intervensi diberikan selama 7 dan 15 hari. Data dianalisis statistik menggunakan uji Two Way Annova. EDPK terbukti dapat menurunkan kadar GDP dan HOMA-IR, serta meningkatkan insulin dan HOMA-B pada tikus jantan wistar model diabetes melitus yang diinduksi STZ+NA . EDPK dengan dosis 300 mg/kg BB/hari intervensi terbukti memiliki efek antihipeglikemi paling kuat.

Keywords


patikan kebo leaves extract; blood glucose; insulin levels; Homa-IR; Homa-B

References


Ajie, R. B. (2015). White Dragon Fruit (Hylocereus undatus) Potential As Diabetes Melitus Treatment. Journal Majority, 4(1), 69–72.

Akbarzadeh, A. (2007). Induction of Diabetes by Streptozoticin in Rats. Indian Journal, 22(2), 60–64.

Al-Ishaq, R. K., Abotaleb, M., Kubatka, P., Kajo, K., & Büsselberg, D. (2019). Flavonoids and their anti-diabetic effects: Cellular mechanisms and effects to improve blood sugar levels. Biomolecules, 9(9). https://doi.org/10.3390/biom9090430

Asha S, Deevika B & Sadiq, MA. (2014). Euphorbia Hirta Linn- A Review On Traditional Uses. World Journal Phytochemistry and Pharmacology. 3(4), 180–205. https://doi.org/10.11648/j.ijde.20170203.11

Byun A R, Lee WA, Lee HS, Shim KW. (2015). What is the most appropriate lipid profile ratio predictor for insulin resistance in each sex? A cross-sectional study in Korean populations (The Fifth Korea National Health and Nutrition Examination Survey). Diabetology and metabolic syndrome, 28(7):59.

Chandran, R., Primelazhagan, T., Shanmugam, S., & Thankarajan, S. (2016). Antidiabetic activity of Syzygium calophyllifolium in Streptozotocin-Nicotinamide induced Type-2 diabetic rats. Biomedicine and Pharmacotherapy, 82, 547–554. https://doi.org/10.1016/j.biopha.2016.05.036

Delgado, G. T. C., Tamashiro, W. M. da S. C., Junior, M. R. M., & Pastore, G. M. (2013). Yacon (Smallanthus sonchifolius): A Functional Food. Plant Foods for Human Nutrition, 68(3), 222–228. https://doi.org/10.1007/s11130-013-0362-0

Devi, S., & Kumar, M. (2017). To cite this article: Sangeeta Devi, Muneesh Kumar. In-vivo Antidiabetic Activity of Methanolic Extract of Euphorbia hirta L. International Journal of Diabetes and Endocrinology, 2(3), 36–39. https://doi.org/10.11648/j.ijde.20170203.11

Furman BL. 2015. Streptozotocin Induced Diabetic Models in Mice and Rats. Current Protocols in Pharmacology., Vol. 70, p. 5.47.1-5.47.20. https://doi.org/10.1002/0471141755.ph0547s70.

Ghasemi, A., Khalifi, S., & Jedi, S. (2014). Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). 101(4), 408–420. https://doi.org/10.1002/0471141755.ph0547s70.

Goud, B. J. (2015). Streptozotocin—A Diabetogenic Agent in Animal Models. International Journal of Pharmacy & Pharmaceuticals Research. 3(1): 253–269

IDF. (2017). Diabetes Atlas Five edition, 5th ed. International Diabetes Federation (IDF).

Jadhav, R., & Puchchakayala, G. (2011). Hypoglicemic and Antidiabetic Activity Of Flavonoids: Boswellic Acid, Ellagic Acid, Quercetin, Rutin On STZ-NA Induced Type 2 Diabetic Rats. International Journal Pharm Pharmaceut Sci ., 2 (2) : 231-256. https://www.researchgate.net/publication/279675150_Hypoglycemic_and_antidiabetic_activity_of_flavonoids_Boswellic_acid_Ellagic_acid_Quercetin_Rutin_on_streptozotocin-nicotinamide_induced_type_2_diabetic_rats

Kemenkes Republik Indonesia. (2018). Riset Kesehatan Dasar (Riskesdas) 2018. Kementrian Kesehatan Indonesia.

Kemenkes Republik Indonesia. 2017. Gizi Dalam Daur Kehidupan. Jakarta: Kemenkes Republik Indonesia.

Kurniawati, D. M., Dharmana, E., Rachmawati, B., & Pemayun, T. G. D. (2017). Pengaruh pemberian ekstrak kedelai hitam (Glycine soja.) berbagai dosis terhadap kadar glukosa darah, kadar insulin, dan HOMA-IR. The Indonesian Journal of Nutrition, 6(1), 44–50. https://doi.org/10.14710/jgi.6.1.44-50

Nugroho, W. (2017). Efek Pemberian Ekstrak Etanol Daun Jarak Merah Terhadap HOMA-IR, HOMA-?, dan aktivitas Ppar? SEl Adiposit Model Tikus DM Tipe 2. Thesis. Universitas Airlangga.

Nurhidajah and Nurrahman. (2016). Hypoglycemic Effect of Red Rice Germ on Insulin Levels, HOMA-IR and HOMA ? Index of STZ-NA Induced Rats. Agritech 36(4), 433–439. http://dx.doi.org/10.22146/agritech.16767

Omiya, K., Minami, K., Sato, Y., Takai, M., Takahashi, E., Hayashi, A., Yamauchi, M., Suzuki, K., Akashi, Y. J., Osada, N., Izawa, K. P., & Watanabe, S. (2015). Impaired ?-cell function attenuates training effects by reducing the increase in heart rate reserve in patients with myocardial infarction. Journal of Cardiology, 65(2), 128–133. https://doi.org/10.1016/j.jjcc.2014.04.012

Pereira DF, Cazarolli LH, Lavado C, Mengatto V, Figueiredo MS, Guedes A, Pizzolatti MG, Silva FR. Effects Of Flavonoids On ?-Glucosidase Activity: Potencial Targets Of Glucose Homeostatis. Nutrition, 27:1161-7. https://doi.org/10.1016/j.nut.2011.01.008

Pitocco, D., Tesauro, M., Alessandro, R., Ghirlanda, G., & Cardillo, C. (2013). Oxidative Stress in Diabetes: Implications for Vascular and Other Complications. International Journal of Molecular Sciences, 14(11), 21525–21550. https://doi.org/10.3390/ijms141121525

Shai, L. J., Masoko, P., Mokgotho, M. P., Magano, S. R., Mogale, A. M., Boaduo, N., & Eloff, J. N. (2010). Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South African Journal of Botany, 76(3), 465–470. https://doi.org/10.1016/j.sajb.2010.03.002

Sheliya, M. A., Rayhana, B., Ali, A., Pillai, K. K., Aeri, V., Sharma, M., & Mir, S. R. (2015). Inhibition of ?-glucosidase by new prenylated flavonoids from euphorbia hirta L. herb. Journal of Ethnopharmacology, 176, 1–8. https://doi.org/10.1016/j.jep.2015.10.018

Shen, L., Keenan, M. J., Raggio, A., Williams, C., & Martin, R. J. (2011). Dietary-resistant starch improves maternal glycemic control in Goto-Kakizaki rat. Molecular Nutrition and Food Research, 55(10), 1499–1508. https://doi.org/10.1002/mnfr.201000605

Stevani, H. (2016). Praktikum Farmakologi 1st ed. Pusdik SDM Kesehatan.

Subiyono, Martsiningsih M.A, D. G. S. (2016). Gambaran Kadar Glukosa Darah Metode GOD-PAP (Glucose Oxsidase-Peroxidase Aminoantypirin) Sampel Serum dan Plasma EDTA (Ethylen Diamin Terta Acetat) (Vol. 5, Issue 1, pp. 2338–5634). Gambaran Kadar Glukosa Darah. www.teknolabjournal.com

Szkudelski, T., Zywert, A., & Szkudelska, K. (2013). Metabolic disturbances and defects in insulin secretion in rats with streptozotocin-nicotinamide-induced diabetes. Physiological Research, 62(6), 663–670. https://doi.org/10.33549/physiolres.932509

Tran N., Nguye M, T. Q. & L. L. (2020). Screening of antibacterial activity, antioxidant activity, and anticancer activity of euphorbia hirta linn. Extracts. Applied Sciences (Switzerland), 10(23), 1–15. https://doi.org/10.3390/app10238408

Tran, N., Tran, M., Truong, H., & Le, L. (2020). Spray-Drying Microencapsulation of High Concentration of Bioactive Compounds Fragments from Euphorbia hirta L. Extract and Their Effect on Diabetes Mellitus. Foods, 9(7). https://doi.org/10.3390/foods9070881

Unger RH & Orchi L. (2010). Paracrinology of islets and the paracrinopathy of diabetes. Proceedings of the National Academy of Sciences, 107(37): 16009–16012. https://doi.org/10.1073/pnas.1006639107

Zahra, F., Budhiarta, A. A. G., & Pangkahila, W. (2017). Pemberian ekstrak daun cincau (Mesona palustris BL) oral meningkatkan jumlah sel ? pankreas dan menurunkan gula darah puasa pada tikus putih (Rattus norvegicus) jantan galur Wistar diabetes. In Jurnal e-Biomedik (eBm) (Vol. 5, Issue 1). https://doi.org/10.35790/ebm.v5i1.15034


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